Composite

Part:BBa_K3002234:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 hygromycin resistance 2 + cCA_Mut-PETase_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2633
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1452
    Illegal NgoMIV site found at 3188
    Illegal NgoMIV site found at 3215
    Illegal NgoMIV site found at 4986
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002127 (hygromycin resistance 2) and BBa_K3002109 (cCA_mutant PETase_SP20HA).

References